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معمل الكروماتجرافي
 
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GC/Mass Spectrometer

An important analytical technique composed of two parts, gas chromatography (GC) and mass spectrometry(MS), which have been compined.
By passing a mixture(sample) in gas stream (Helium) through along capillary column with inside walls thinly coated with stationary phase, the components of the mixture separate and emerge one after another.
Separated components are detected and recorded using mass spectrometry.

Application:

Identification of reaction products by synthetic organic chemists.
• Analysis of industrial products for quality control.
• Determination of molecular weights of unknown organic compounds in complex mixtures.
• Structural determination of unknown organic compounds in complex mixtures by matching their spectra with spectral interpenetration.
• Quantification of drugs and their metabolites in blood and urine for pharmacological application.
• identification of unknown organic compounds extracted from plants and fungies .
 
  Amino Acid analyzer
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  • Amino acid analysis is a technique based on ion exchange liquid chromatography, used in a wide range of application areas to provide qualitative and quantitative compositional analysis.
  • The Amino Acid Analyzer's innovative design leads to both, a high sensitivity and a high degree of reproducibility. All buffer solutions, as well as the Ninhydrine reagent are stored under inert gas pressure (N2) in a refrigerated cabinet to avoid oxidation and air contamination independent of the ambient environment.
  • Integrated Autosampler with a capacity of 120 vials in a cooled sample tray. Volumes from 1μl to 100 μl can be injected without any loss of sample.
  • In general, two different buffer systems are used for the elution of amino acids. Sodium salt buffers are used for the elution of amino acids resulting from protein and peptide hydrolysates. Lithium salt buffers in physiological fluids are used for the determination of free amino acids.
Two different method types can be used

    • Hydrolysate: Apply the qualitative and quantitative analysis of 18 amino acids; (Aspartic acid, Therionine, Serine, Glutamic acid, Proline, Glycine, Alanine, Cystine, Valine, Methionine, Isoleucine, Leucine, Tyrosine, Phenylalanine, Histidine, Lysine, Ammonia  and Argenine).
 
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